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The rules are fewer today (it's okay to wear a hat in the presence of a woman, for example), but not knowing when to take your hat off can make you appear rude, unsophisticated or both. Here are the basic rules of hat etiquette:" Hats traditionally thought of as "women's hats," with ribbons, bows, feathers, flowers, or other ornamentation, or in delicate colors and materials, including the small hats worn on the front of the head and known as fascinators, may be kept on indoors for all occasions.

which creates thymidine monophosphate (TMP) from deoxyuridine monophosphate (dUMP). By additional phosphorylation reactions, TMP can be used to make thymidine triphosphate (TTP), one of the four nucleotide precursors that are used by DNA polymerases to create DNA . Without the THF required to convert dUMP, there can be no TTP, and DNA synthesis cannot proceed, unless TMP can be produced from another source. The alternative source is the thymidine present in the HAT medium that can be absorbed by the cells and phosphorylated by thymidine kinase (TK) into TMP.

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